Device

Part:BBa_I766669:Design

Designed by: Jimmy Jie Min Huang   Group: iGEM07_UCSF   (2007-08-16)


YopJ-RR under weak constitutive promotor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 93
    Illegal PstI site found at 1852
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1852
    Illegal NotI site found at 2411
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 901
    Illegal BamHI site found at 742
    Illegal BamHI site found at 2394
    Illegal XhoI site found at 1492
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 93
    Illegal PstI site found at 1852
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 93
    Illegal PstI site found at 1852
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 670
    Illegal SapI.rc site found at 694


Design Notes

Device was created using a method of combinatorial cloning using the type IIs restriction endonuclease AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.


Source

Composite part.

References